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tzm bl hrp 8129 cells  (ATCC)


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    ATCC tzm bl hrp 8129 cells
    Tzm Bl Hrp 8129 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 405 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tzm+bl/J-111+Human+T-cell+LEUKEMIA+cell/pm41592176-281-3-9
    Average 96 stars, based on 405 article reviews
    tzm bl hrp 8129 cells - by Bioz Stars, 2026-10
    96/100 stars

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    Modification:

    Article Title: Deubiquitinase ubiquitin-specific protease 3 (USP3) inhibits HIV-1 replication via promoting APOBEC3G (A3G) expression in both enzyme activity-dependent and -independent manners
    Article Snippet: [43,44] Cell culture, transfection, and antibodies Jurkat (TIB-152, American Type Culture Collection [ATCC] and H9 (CRM-CCL-2, ATCC) cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium (1640, HyClone, Logan, UT, USA) with 10% fetal bovine serum (FBS, 04-001-1, Biological Industries) and 100 mg/mL penicillin/streptomycin. .. HEK293T (CRL-11268, ATCC), HepG2 (HB-8065, ATCC), and TZM-bl (PTA-5659, ATCC) cells were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA) and maintained in Dulbecco’s modified Eagle medium (DMEM, HyClone, Logan, UT, USA) containing 10% fetal bovine serum (FBS, PAN Seratech, Aidenbach, Germany) and 100mg/mL penicillin/ streptomycin (Biological Industries, Israel), and DNA transfections were carried out by Lipofectamine 2000 (Thermo, Waltham, MA, USA) according to the manufacturer’s instruction. .. H9 cells were transfected using the Amaxa Cell Line Nucleofector Kit V (Lonza, Switzerland) with the program G-014 according to the manufacturer’s instructions.

    Article Title: Deubiquitinase ubiquitin-specific protease 3 (USP3) inhibits HIV-1 replication via promoting APOBEC3G (A3G) expression in both enzyme activity-dependent and -independent manners
    Article Snippet: Jurkat (TIB-152, American Type Culture Collection [ATCC] and H9 (CRM-CCL-2, ATCC) cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium (1640, HyClone, Logan, UT, USA) with 10% fetal bovine serum (FBS, 04-001-1, Biological Industries) and 100 μg/mL penicillin/streptomycin. .. HEK293T (CRL-11268, ATCC), HepG2 (HB-8065, ATCC), and TZM-bl (PTA-5659, ATCC) cells were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA) and maintained in Dulbecco's modified Eagle medium (DMEM, HyClone, Logan, UT, USA) containing 10% fetal bovine serum (FBS, PAN Seratech, Aidenbach, Germany) and 100 μg/mL penicillin/streptomycin (Biological Industries, Israel), and DNA transfections were carried out by Lipofectamine 2000 (Thermo, Waltham, MA, USA) according to the manufacturer's instruction. .. H9 cells were transfected using the Amaxa Cell Line Nucleofector Kit V (Lonza, Switzerland) with the program G-014 according to the manufacturer's instructions.

    Article Title: SARS-CoV-2 variant Alpha has a spike-dependent replication advantage over the ancestral B.1 strain in human cells with low ACE2 expression.
    Article Snippet: .. A549 parental (ATCC CCL-185), ACE2+-A549, TMPRSS2+-A549, ACE2+/TMPRSS2+-A549 [61], Caco-2 (ATCC HTB-37), Calu-3 (HTB-55), CHO (HIV Reagent Program ARP-2238), NCI-H1299 (ATCC CRL-5803), ACE2-KO-NCI-H1299, ACE2+-NCI-H1299, HEK 293T (ATCC CRL-3216), TZM-bl (HIV Reagent Program ARP-8129), and Vero E6 (ATCC CRL1586) cells were maintained at 37 ̊C and 5% CO2 in a humidified atmosphere and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 1% nonessential amino acids 100× concentrate (NEAA, Thermo Fisher Scientific), and 1% sodium pyruvate 100 mM (NaP, Thermo Fisher Scientific) and split twice a week. .. For seeding and cultivation, cells were washed with phosphate buffered saline (PBS, Thermo Fisher Scientific) and detached with 0.05% trypsin-EDTA solution (Thermo Fisher Scientific).

    Article Title: SARS-CoV-2 variant Alpha has a spike-dependent replication advantage over the ancestral B.1 strain in human cells with low ACE2 expression
    Article Snippet: .. A549 parental (ATCC CCL-185), ACE2 + -A549, TMPRSS2 + -A549, ACE2 + /TMPRSS2 + -A549 [ ], Caco-2 (ATCC HTB-37), Calu-3 (HTB-55), CHO (HIV Reagent Program ARP-2238), NCI-H1299 (ATCC CRL-5803), ACE2-KO-NCI-H1299, ACE2 + -NCI-H1299, HEK 293T (ATCC CRL-3216), TZM-bl (HIV Reagent Program ARP-8129), and Vero E6 (ATCC CRL-1586) cells were maintained at 37°C and 5% CO 2 in a humidified atmosphere and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 1% nonessential amino acids 100× concentrate (NEAA, Thermo Fisher Scientific), and 1% sodium pyruvate 100 mM (NaP, Thermo Fisher Scientific) and split twice a week. .. For seeding and cultivation, cells were washed with phosphate buffered saline (PBS, Thermo Fisher Scientific) and detached with 0.05% trypsin-EDTA solution (Thermo Fisher Scientific).

    Article Title: Deubiquitinase USP37 enhances the anti-HIV-2/SIV ability of the host restriction factor SAMHD1.
    Article Snippet: .. HEK293T (CRL-11268; ATCC, Manassas, VA, USA) and TZM-bl (PTA-5659; ATCC) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, South Logan, UT, USA) containing 10% heat-inactivated fetal bovine serum (04-001-1; Biological Industries, Kibbutz Beit Haemek, Israel) and 100 μg/mL penicillin–streptomycin. ..

    Article Title: Macrophages derived from human induced pluripotent stem cells (iPSCs) serve as a high-fidelity cellular model for investigating HIV-1, dengue, and influenza viruses
    Article Snippet: Cell lines and culture conditions Monocytic cell lines THP-1 (ATCC, #TIB-202) and U937 (ATCC, #CRL-1593.2) were cultured in RPMI-1640 medium (ATCC, #30-2001) with 10% FBS and 1% Pen Strep (complete medium). .. TZM-bl (NIH ARP, #8129), MDCK (ATCC, #CCL-34), and BHK (ATCC, #PTA-3544) cells were cultured in Dulbecco’s modified Eagle medium (Sigma-Aldrich, #D6429) with 10% FBS, 2-mM L-glutamine (Corning, #25-005-CI), and 1% Pen Strep (Corning, #MT30002CI) (complete medium). .. A549 cells were cultured with F-12K medium (Corning, #10-025-CV) supplemented with 10% FBS, 2-mM L-glutamine, and 1% Pen Strep.

    Article Title: Deubiquitinase USP37 enhances the anti-HIV-2/SIV ability of the host restriction factor SAMHD1
    Article Snippet: .. HEK293T (CRL-11268; ATCC, Manassas, VA, USA) and TZM-bl (PTA-5659; ATCC) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, South Logan, UT, USA) containing 10% heat-inactivated fetal bovine serum (04-001-1; Biological Industries, Kibbutz Beit Haemek, Israel) and 100 μg/mL penicillin–streptomycin. .. Human monocytic cell line THP-1 (TIB-202; ATCC) cells were maintained in RPMI 1640 medium (RPMI 1640; HyClone) containing 10% fetal bovine serum and 100 μg/mL penicillin–streptomycin.

    Transfection:

    Article Title: Deubiquitinase ubiquitin-specific protease 3 (USP3) inhibits HIV-1 replication via promoting APOBEC3G (A3G) expression in both enzyme activity-dependent and -independent manners
    Article Snippet: [43,44] Cell culture, transfection, and antibodies Jurkat (TIB-152, American Type Culture Collection [ATCC] and H9 (CRM-CCL-2, ATCC) cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium (1640, HyClone, Logan, UT, USA) with 10% fetal bovine serum (FBS, 04-001-1, Biological Industries) and 100 mg/mL penicillin/streptomycin. .. HEK293T (CRL-11268, ATCC), HepG2 (HB-8065, ATCC), and TZM-bl (PTA-5659, ATCC) cells were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA) and maintained in Dulbecco’s modified Eagle medium (DMEM, HyClone, Logan, UT, USA) containing 10% fetal bovine serum (FBS, PAN Seratech, Aidenbach, Germany) and 100mg/mL penicillin/ streptomycin (Biological Industries, Israel), and DNA transfections were carried out by Lipofectamine 2000 (Thermo, Waltham, MA, USA) according to the manufacturer’s instruction. .. H9 cells were transfected using the Amaxa Cell Line Nucleofector Kit V (Lonza, Switzerland) with the program G-014 according to the manufacturer’s instructions.

    Article Title: Deubiquitinase ubiquitin-specific protease 3 (USP3) inhibits HIV-1 replication via promoting APOBEC3G (A3G) expression in both enzyme activity-dependent and -independent manners
    Article Snippet: Jurkat (TIB-152, American Type Culture Collection [ATCC] and H9 (CRM-CCL-2, ATCC) cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium (1640, HyClone, Logan, UT, USA) with 10% fetal bovine serum (FBS, 04-001-1, Biological Industries) and 100 μg/mL penicillin/streptomycin. .. HEK293T (CRL-11268, ATCC), HepG2 (HB-8065, ATCC), and TZM-bl (PTA-5659, ATCC) cells were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA) and maintained in Dulbecco's modified Eagle medium (DMEM, HyClone, Logan, UT, USA) containing 10% fetal bovine serum (FBS, PAN Seratech, Aidenbach, Germany) and 100 μg/mL penicillin/streptomycin (Biological Industries, Israel), and DNA transfections were carried out by Lipofectamine 2000 (Thermo, Waltham, MA, USA) according to the manufacturer's instruction. .. H9 cells were transfected using the Amaxa Cell Line Nucleofector Kit V (Lonza, Switzerland) with the program G-014 according to the manufacturer's instructions.

    Cell Culture:

    Article Title: SARS-CoV-2 variant Alpha has a spike-dependent replication advantage over the ancestral B.1 strain in human cells with low ACE2 expression.
    Article Snippet: .. A549 parental (ATCC CCL-185), ACE2+-A549, TMPRSS2+-A549, ACE2+/TMPRSS2+-A549 [61], Caco-2 (ATCC HTB-37), Calu-3 (HTB-55), CHO (HIV Reagent Program ARP-2238), NCI-H1299 (ATCC CRL-5803), ACE2-KO-NCI-H1299, ACE2+-NCI-H1299, HEK 293T (ATCC CRL-3216), TZM-bl (HIV Reagent Program ARP-8129), and Vero E6 (ATCC CRL1586) cells were maintained at 37 ̊C and 5% CO2 in a humidified atmosphere and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 1% nonessential amino acids 100× concentrate (NEAA, Thermo Fisher Scientific), and 1% sodium pyruvate 100 mM (NaP, Thermo Fisher Scientific) and split twice a week. .. For seeding and cultivation, cells were washed with phosphate buffered saline (PBS, Thermo Fisher Scientific) and detached with 0.05% trypsin-EDTA solution (Thermo Fisher Scientific).

    Article Title: SARS-CoV-2 variant Alpha has a spike-dependent replication advantage over the ancestral B.1 strain in human cells with low ACE2 expression
    Article Snippet: .. A549 parental (ATCC CCL-185), ACE2 + -A549, TMPRSS2 + -A549, ACE2 + /TMPRSS2 + -A549 [ ], Caco-2 (ATCC HTB-37), Calu-3 (HTB-55), CHO (HIV Reagent Program ARP-2238), NCI-H1299 (ATCC CRL-5803), ACE2-KO-NCI-H1299, ACE2 + -NCI-H1299, HEK 293T (ATCC CRL-3216), TZM-bl (HIV Reagent Program ARP-8129), and Vero E6 (ATCC CRL-1586) cells were maintained at 37°C and 5% CO 2 in a humidified atmosphere and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 1% nonessential amino acids 100× concentrate (NEAA, Thermo Fisher Scientific), and 1% sodium pyruvate 100 mM (NaP, Thermo Fisher Scientific) and split twice a week. .. For seeding and cultivation, cells were washed with phosphate buffered saline (PBS, Thermo Fisher Scientific) and detached with 0.05% trypsin-EDTA solution (Thermo Fisher Scientific).

    Article Title: Macrophages derived from human induced pluripotent stem cells (iPSCs) serve as a high-fidelity cellular model for investigating HIV-1, dengue, and influenza viruses
    Article Snippet: Cell lines and culture conditions Monocytic cell lines THP-1 (ATCC, #TIB-202) and U937 (ATCC, #CRL-1593.2) were cultured in RPMI-1640 medium (ATCC, #30-2001) with 10% FBS and 1% Pen Strep (complete medium). .. TZM-bl (NIH ARP, #8129), MDCK (ATCC, #CCL-34), and BHK (ATCC, #PTA-3544) cells were cultured in Dulbecco’s modified Eagle medium (Sigma-Aldrich, #D6429) with 10% FBS, 2-mM L-glutamine (Corning, #25-005-CI), and 1% Pen Strep (Corning, #MT30002CI) (complete medium). .. A549 cells were cultured with F-12K medium (Corning, #10-025-CV) supplemented with 10% FBS, 2-mM L-glutamine, and 1% Pen Strep.



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    a Experimental workflow. HIV-1 NL4.3 virions were cultured in suspension (S) or embedded in collagen matrices (DC: Dense collagen; LC: Loose <t>collagen).</t> <t>TZM-bl</t> cells were infected and the relative infectivity determined. b Normalized infectivity of HIV-1 NL4.3 particles. Virions were cultured in S, DC or LC for 16 h prior to relative infectivity determination. Data is normalized to suspension condition (gray dotted line). c Kinetics of ERVI. Culture supernatants were harvested at 0, 4, 8 or 16 h post seeding/embedding, the relative infectivity of virions was determined as in b . d Normalized infectivity of differentially cultured HIV-1 NL4.3 virions. PA: polymerizing agent. e . One representative Western Blot analysis showing the gp120/p24 ratio from HIV-1 NL4.3 virions cultured in S, DC or LC. The gp120/p24 ratios are indicated below the blots. f Quantification of western blot analyses from three independent experiments as in e . g Saturation of ERVI. Increasing amounts of virions were cultured in S, DC or LC prior to infectivity determination. h Normalized infectivity of HIV-1 NL4.3 particles after seeding or embedding in different matrices for 16 h. Data are normalized to suspension condition (gray dotted line). i Conservation of sensitivity to ERVI across lentiviruses. The normalized infectivity of a panel of lentiviruses was assessed after ERVI as in a . Data are normalized to respective suspension condition (gray dotted line). Results represent the mean ± SD of three independent experiments. Symbols indicate data from individual experiments. Significance is indicated by p -values, and was calculated by one-way ANOVA test; Dunnett’s post-test ( b , d , h , i ), or by matched two-way ANOVA test; Tukey’s post-test ( g ). Source data are provided as a file.
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    99
    ATCC n a tzm bl bei resources
    a Experimental workflow. HIV-1 NL4.3 virions were cultured in suspension (S) or embedded in collagen matrices (DC: Dense collagen; LC: Loose <t>collagen).</t> <t>TZM-bl</t> cells were infected and the relative infectivity determined. b Normalized infectivity of HIV-1 NL4.3 particles. Virions were cultured in S, DC or LC for 16 h prior to relative infectivity determination. Data is normalized to suspension condition (gray dotted line). c Kinetics of ERVI. Culture supernatants were harvested at 0, 4, 8 or 16 h post seeding/embedding, the relative infectivity of virions was determined as in b . d Normalized infectivity of differentially cultured HIV-1 NL4.3 virions. PA: polymerizing agent. e . One representative Western Blot analysis showing the gp120/p24 ratio from HIV-1 NL4.3 virions cultured in S, DC or LC. The gp120/p24 ratios are indicated below the blots. f Quantification of western blot analyses from three independent experiments as in e . g Saturation of ERVI. Increasing amounts of virions were cultured in S, DC or LC prior to infectivity determination. h Normalized infectivity of HIV-1 NL4.3 particles after seeding or embedding in different matrices for 16 h. Data are normalized to suspension condition (gray dotted line). i Conservation of sensitivity to ERVI across lentiviruses. The normalized infectivity of a panel of lentiviruses was assessed after ERVI as in a . Data are normalized to respective suspension condition (gray dotted line). Results represent the mean ± SD of three independent experiments. Symbols indicate data from individual experiments. Significance is indicated by p -values, and was calculated by one-way ANOVA test; Dunnett’s post-test ( b , d , h , i ), or by matched two-way ANOVA test; Tukey’s post-test ( g ). Source data are provided as a file.
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    a Experimental workflow. HIV-1 NL4.3 virions were cultured in suspension (S) or embedded in collagen matrices (DC: Dense collagen; LC: Loose collagen). TZM-bl cells were infected and the relative infectivity determined. b Normalized infectivity of HIV-1 NL4.3 particles. Virions were cultured in S, DC or LC for 16 h prior to relative infectivity determination. Data is normalized to suspension condition (gray dotted line). c Kinetics of ERVI. Culture supernatants were harvested at 0, 4, 8 or 16 h post seeding/embedding, the relative infectivity of virions was determined as in b . d Normalized infectivity of differentially cultured HIV-1 NL4.3 virions. PA: polymerizing agent. e . One representative Western Blot analysis showing the gp120/p24 ratio from HIV-1 NL4.3 virions cultured in S, DC or LC. The gp120/p24 ratios are indicated below the blots. f Quantification of western blot analyses from three independent experiments as in e . g Saturation of ERVI. Increasing amounts of virions were cultured in S, DC or LC prior to infectivity determination. h Normalized infectivity of HIV-1 NL4.3 particles after seeding or embedding in different matrices for 16 h. Data are normalized to suspension condition (gray dotted line). i Conservation of sensitivity to ERVI across lentiviruses. The normalized infectivity of a panel of lentiviruses was assessed after ERVI as in a . Data are normalized to respective suspension condition (gray dotted line). Results represent the mean ± SD of three independent experiments. Symbols indicate data from individual experiments. Significance is indicated by p -values, and was calculated by one-way ANOVA test; Dunnett’s post-test ( b , d , h , i ), or by matched two-way ANOVA test; Tukey’s post-test ( g ). Source data are provided as a file.

    Journal: Nature Communications

    Article Title: A tissue-intrinsic mechanism sensitizes HIV-1 particles for TLR-triggered innate immune responses

    doi: 10.1038/s41467-026-72586-3

    Figure Lengend Snippet: a Experimental workflow. HIV-1 NL4.3 virions were cultured in suspension (S) or embedded in collagen matrices (DC: Dense collagen; LC: Loose collagen). TZM-bl cells were infected and the relative infectivity determined. b Normalized infectivity of HIV-1 NL4.3 particles. Virions were cultured in S, DC or LC for 16 h prior to relative infectivity determination. Data is normalized to suspension condition (gray dotted line). c Kinetics of ERVI. Culture supernatants were harvested at 0, 4, 8 or 16 h post seeding/embedding, the relative infectivity of virions was determined as in b . d Normalized infectivity of differentially cultured HIV-1 NL4.3 virions. PA: polymerizing agent. e . One representative Western Blot analysis showing the gp120/p24 ratio from HIV-1 NL4.3 virions cultured in S, DC or LC. The gp120/p24 ratios are indicated below the blots. f Quantification of western blot analyses from three independent experiments as in e . g Saturation of ERVI. Increasing amounts of virions were cultured in S, DC or LC prior to infectivity determination. h Normalized infectivity of HIV-1 NL4.3 particles after seeding or embedding in different matrices for 16 h. Data are normalized to suspension condition (gray dotted line). i Conservation of sensitivity to ERVI across lentiviruses. The normalized infectivity of a panel of lentiviruses was assessed after ERVI as in a . Data are normalized to respective suspension condition (gray dotted line). Results represent the mean ± SD of three independent experiments. Symbols indicate data from individual experiments. Significance is indicated by p -values, and was calculated by one-way ANOVA test; Dunnett’s post-test ( b , d , h , i ), or by matched two-way ANOVA test; Tukey’s post-test ( g ). Source data are provided as a file.

    Article Snippet: 293T cells (ATCC, CRL-3216) and TZM-bl reporter cells (NIH AIDS Reagent Program, courtesy of Dr. John C. Kappes, Dr. Xiaoyun Wu and Tranzyme Inc. (ARP-8129)) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% heat-inactivated fetal calf serum (FCS, Capricorn) and 1% penicillin/streptomycin (Gibco).

    Techniques: Cell Culture, Suspension, Infection, Western Blot

    a Normalized infectivity of HIV-1 NL4.3 virus containing Vpr.Int.GFP fusion proteins after exposure to LC. b Experimental workflow to assess collagen deposition at the surface of virions. HIV-1 NL4.3 Vpr.Int.GFP virions were collected from S, LC or LCAF647 cultures and analyzed by microscopy. c Representative confocal micrographs of HIV1 NL4.3 Vpr.Int.GFP virions within AlexaFluor 647 stained LC matrices. Yellow arrows depict fluorescent virions. Scale bar: 20 µm. d Representative TIRF-micrographs of HIV-1 NL4.3 Vpr.Int.GFP virions were harvested from the supernatants of fluorescently labeled collagen matrices as in ( c ). Scale bar: 5 µm. e Averaged computational slices of a tomogram showing HIV-1 NL4.3 virions after 16 h of culture in S (left panel), DC (middle panel), or LC (right panel). Scale bar = 50 nm. f Quantification of the diameter of virus particles treated as indicated in d . Violin plot shows individual data points with corresponding median, 25% and 75% quartiles. g Averaged computational slices of a tomogram showing disrupted dense (left panel) or loose (right panel) collagen fibers. Scale bar = 100 nm. h Experimental workflow. HIV-1 NL4.3 virions retrieved from suspension or collagen matrices after 16 h of culture were harvested, and equivalent amounts of RT units were incubated with RM-8 or EF-C infectivity-enhancing peptide, nanofibrils (PNFs) or left untreated (UT) prior to infection of TZM-bl reporter cells. i Impact of infectivity enhancers on the infectivity of suspension or collagen primed virions. Results represent the mean ± SD of three independent experiments. Symbols indicate individual experiments. Significance is indicated by p -values, and was calculated by one-way ANOVA, Dunnett’s post-test ( a ), unpaired t-tests ( d ), or by two-way ANOVA test with Geisser Greenhouse correction; Tukey’s post-test ( i ). Source data are provided as a file.

    Journal: Nature Communications

    Article Title: A tissue-intrinsic mechanism sensitizes HIV-1 particles for TLR-triggered innate immune responses

    doi: 10.1038/s41467-026-72586-3

    Figure Lengend Snippet: a Normalized infectivity of HIV-1 NL4.3 virus containing Vpr.Int.GFP fusion proteins after exposure to LC. b Experimental workflow to assess collagen deposition at the surface of virions. HIV-1 NL4.3 Vpr.Int.GFP virions were collected from S, LC or LCAF647 cultures and analyzed by microscopy. c Representative confocal micrographs of HIV1 NL4.3 Vpr.Int.GFP virions within AlexaFluor 647 stained LC matrices. Yellow arrows depict fluorescent virions. Scale bar: 20 µm. d Representative TIRF-micrographs of HIV-1 NL4.3 Vpr.Int.GFP virions were harvested from the supernatants of fluorescently labeled collagen matrices as in ( c ). Scale bar: 5 µm. e Averaged computational slices of a tomogram showing HIV-1 NL4.3 virions after 16 h of culture in S (left panel), DC (middle panel), or LC (right panel). Scale bar = 50 nm. f Quantification of the diameter of virus particles treated as indicated in d . Violin plot shows individual data points with corresponding median, 25% and 75% quartiles. g Averaged computational slices of a tomogram showing disrupted dense (left panel) or loose (right panel) collagen fibers. Scale bar = 100 nm. h Experimental workflow. HIV-1 NL4.3 virions retrieved from suspension or collagen matrices after 16 h of culture were harvested, and equivalent amounts of RT units were incubated with RM-8 or EF-C infectivity-enhancing peptide, nanofibrils (PNFs) or left untreated (UT) prior to infection of TZM-bl reporter cells. i Impact of infectivity enhancers on the infectivity of suspension or collagen primed virions. Results represent the mean ± SD of three independent experiments. Symbols indicate individual experiments. Significance is indicated by p -values, and was calculated by one-way ANOVA, Dunnett’s post-test ( a ), unpaired t-tests ( d ), or by two-way ANOVA test with Geisser Greenhouse correction; Tukey’s post-test ( i ). Source data are provided as a file.

    Article Snippet: 293T cells (ATCC, CRL-3216) and TZM-bl reporter cells (NIH AIDS Reagent Program, courtesy of Dr. John C. Kappes, Dr. Xiaoyun Wu and Tranzyme Inc. (ARP-8129)) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% heat-inactivated fetal calf serum (FCS, Capricorn) and 1% penicillin/streptomycin (Gibco).

    Techniques: Infection, Virus, Microscopy, Staining, Labeling, Suspension, Incubation

    a Experimental workflow. HIV-1 NL4.3 Vpr.mRuby2 virions were cultured in S, DC or LC for 16 h. Equivalent amounts of RT units from culture supernatants or untreated virions were then incubated with TZM-bl cells for 2 h at 4 °C. Cell membranes were then stained with Concanavalin-A AF-488, prior to microscopy processing. b Normalized infectivity of HIV-1 NL4.3 Vpr.mRuby2 virions after 16 h of culture as in a . c Representative spinning disk micrographs of cells incubated with HIV-1 NL4.3 Vpr.mRuby2 virions. Yellow arrows indicate Vpr.mRuby2+ spots detected at the surface of the target cells. Scale bar: 15 µm. d Average binding frequency of HIV-1 NL4.3 Vpr.mRuby2 virions to target cells as shown in c . e Average volume of Vpr.mRuby2 spots detected as shown in c . f Experimental workflow. HIV1 NL4.3 Vpr.BlaM virions were as in a . Equivalent amounts of RT units from culture supernatants or untreated virions were then incubated with TZM-bl cells for 4 h at 37 °C, in the presence or absence of T20 fusion inhibitor. Cells were then loaded with the CCF2-AM and processed by flow cytometry. g Normalized infectivity of HIV-1 NL4.3 Vpr.BlaM virions after16h of culture as in a . h Representative flow cytometry dot plots of CCF2 loaded cells. Gates identify cleaved-CCF2+ cells. See Sup. Figure. for gating strategy. i Quantification of the percentage of CCF2-product positive cells measured by flow cytometry. j Correlation between Relative Infectivity and levels of CCF2-product positive cells after ERVI by linear regression. Results represent the mean ± SD of three independent experiments. Symbols indicate individual experiments. Significance is indicated by p -values, and was calculated by one-way ANOVA test; Dunnets’s post-test ( b , g ), or by one-way ANOVA test; Tukey’s post-test ( d , e , i ). Source data are provided as a file.

    Journal: Nature Communications

    Article Title: A tissue-intrinsic mechanism sensitizes HIV-1 particles for TLR-triggered innate immune responses

    doi: 10.1038/s41467-026-72586-3

    Figure Lengend Snippet: a Experimental workflow. HIV-1 NL4.3 Vpr.mRuby2 virions were cultured in S, DC or LC for 16 h. Equivalent amounts of RT units from culture supernatants or untreated virions were then incubated with TZM-bl cells for 2 h at 4 °C. Cell membranes were then stained with Concanavalin-A AF-488, prior to microscopy processing. b Normalized infectivity of HIV-1 NL4.3 Vpr.mRuby2 virions after 16 h of culture as in a . c Representative spinning disk micrographs of cells incubated with HIV-1 NL4.3 Vpr.mRuby2 virions. Yellow arrows indicate Vpr.mRuby2+ spots detected at the surface of the target cells. Scale bar: 15 µm. d Average binding frequency of HIV-1 NL4.3 Vpr.mRuby2 virions to target cells as shown in c . e Average volume of Vpr.mRuby2 spots detected as shown in c . f Experimental workflow. HIV1 NL4.3 Vpr.BlaM virions were as in a . Equivalent amounts of RT units from culture supernatants or untreated virions were then incubated with TZM-bl cells for 4 h at 37 °C, in the presence or absence of T20 fusion inhibitor. Cells were then loaded with the CCF2-AM and processed by flow cytometry. g Normalized infectivity of HIV-1 NL4.3 Vpr.BlaM virions after16h of culture as in a . h Representative flow cytometry dot plots of CCF2 loaded cells. Gates identify cleaved-CCF2+ cells. See Sup. Figure. for gating strategy. i Quantification of the percentage of CCF2-product positive cells measured by flow cytometry. j Correlation between Relative Infectivity and levels of CCF2-product positive cells after ERVI by linear regression. Results represent the mean ± SD of three independent experiments. Symbols indicate individual experiments. Significance is indicated by p -values, and was calculated by one-way ANOVA test; Dunnets’s post-test ( b , g ), or by one-way ANOVA test; Tukey’s post-test ( d , e , i ). Source data are provided as a file.

    Article Snippet: 293T cells (ATCC, CRL-3216) and TZM-bl reporter cells (NIH AIDS Reagent Program, courtesy of Dr. John C. Kappes, Dr. Xiaoyun Wu and Tranzyme Inc. (ARP-8129)) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% heat-inactivated fetal calf serum (FCS, Capricorn) and 1% penicillin/streptomycin (Gibco).

    Techniques: Cell Culture, Incubation, Staining, Microscopy, Infection, Binding Assay, Flow Cytometry